In this program, we'll look at the production of Green Fluorescent Protein. GFP is used as a biological marker, and when attached to a drug it can provide researchers with a visual story of where the drug goes. It's a fluorescent dye that's very well tolerated by most cells and doesn't interfere with normal cellular function. Let's examine the 3 main phases of GFP production – Fermentation, Recovery, and Purification. Fermentation is basically cell farming. We program cells to produce a product, we nurture them as they grow and reproduce, and then we harvest them. In recovery we separate our product from the cells where they were housed. And then in purification we go a step further by removing everything else that's contaminating our product leaving us with a very pure, concentrated solution.

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Change as an Opportunity
New Experiences
Considering Others' Viewpoints
Dealing with Stress

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Understanding Different Points of View
Understanding the Context of Problems
Connecting Information with Critical Thinking
Thinking About Multiple Solutions

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Working as aTeam Player
Finding Common Ground
Shared Responsibilities and Creative Input
Dealing with Conflict in a Positive Way

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Speaking Professionally
Communicating Digitally
Nonverbal Communication
Understanding How to Listen

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Inspiring Collaboration
Ethics and Innovation
Utilizing Technological Advances
Searching for Information

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Differentiating Empathy with Sympathy
Actively Listening
Respecting Diverse Backgrounds
Understanding Others' Needs at Work

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Taking Initiative
Connecting Ideas and Innovating
Taking Risks and Learning from Mistakes
Thinking Like an Entrepreneur

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Being Prepared
Bouncing Back and Learning from Adversity
Dealing with Conflict and Compromise
Seeing Personal Growth as a Way of Life

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Evaluating Strengths and Areas of Growth
Understanding the Effects of Actions
Workplace Manners
Finding a Good Fit

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Respecting Other Backgrounds
Understanding the Importance of Social Diversity
Expanding Your Definition of "Normal"
Working Effectively in a Diverse Environment

After the second homogenization, the lysed cell solution is pumped back through the centrifuge. But this time, our goal is different. Before lysing, our product was held within the E.coli cells. Now – with the cells broken apart, the cell contents - including cytoplasm and GFP - are mixed into the buffered solution. The centrifuge again spins out the solids - which are primarily cell debris - and it's the clarified liquid which contains our product – the GFP! This time we discard the solids and keep the liquid – which is now known as Lysate.

The video resource "3D Graphics: Crash Course Computer Science #27" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

Different cells have different needs. Some are aerobic – they need oxygen – while others are anaerobic and don't need oxygen. But the lifecycle of almost all cells follows a predictable 4 phase-pattern. Lag, Exponential, Stationary and Death. When a cell is first introduced to fresh growth media, it has to adapt to the new environment. This creates a lag in the growth timeline. After the organism adapts, the batch takes off. The cells begin dividing at a constant rate –an Exponential or Logarithmic increase. But eventually, the nutrients in the media are consumed, toxic metabolic waste products build up, cells begin to die, and growth slows. When just as many cells are dying as are dividing, the batch enters the Stationary phase. This is the point at which the key nutrients are completely consumed, the fermentation is stopped and the broth is harvested. If the fermentation were allowed to continue, the cells would enter the Death phase.

The video resource "Advanced CPU Designs: Crash Course Computer Science #9" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

After catastrophic flooding in New Orleans destroyed two hospitals, the Southeast Louisiana Veterans Health Care System is planning a replacement facility that will incorporate resilience against future extreme events.

Widespread damage from flooding at the Texas Medical Center in Houston revealed the complex's vulnerabilities. Implementing a long-term hazard mitigation plan is reducing future risks.

The video resource "Alan Turing: Crash Course Computer Science #15" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

This site deals with issues related to individual rights and liberties. The site provides up-to-date cases that can be used for for the enhancement of criminal law and constitutional law students' research.