In this program, we'll look at the production of Green Fluorescent Protein. GFP is used as a biological marker, and when attached to a drug it can provide researchers with a visual story of where the drug goes. It's a fluorescent dye that's very well tolerated by most cells and doesn't interfere with normal cellular function. Let's examine the 3 main phases of GFP production – Fermentation, Recovery, and Purification. Fermentation is basically cell farming. We program cells to produce a product, we nurture them as they grow and reproduce, and then we harvest them. In recovery we separate our product from the cells where they were housed. And then in purification we go a step further by removing everything else that's contaminating our product leaving us with a very pure, concentrated solution.
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After the second homogenization, the lysed cell solution is pumped back through the centrifuge. But this time, our goal is different. Before lysing, our product was held within the E.coli cells. Now – with the cells broken apart, the cell contents - including cytoplasm and GFP - are mixed into the buffered solution. The centrifuge again spins out the solids - which are primarily cell debris - and it's the clarified liquid which contains our product – the GFP! This time we discard the solids and keep the liquid – which is now known as Lysate.
The video resource "3D Graphics: Crash Course Computer Science #27" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.
Different cells have different needs. Some are aerobic – they need oxygen – while others are anaerobic and don't need oxygen. But the lifecycle of almost all cells follows a predictable 4 phase-pattern. Lag, Exponential, Stationary and Death. When a cell is first introduced to fresh growth media, it has to adapt to the new environment. This creates a lag in the growth timeline. After the organism adapts, the batch takes off. The cells begin dividing at a constant rate –an Exponential or Logarithmic increase. But eventually, the nutrients in the media are consumed, toxic metabolic waste products build up, cells begin to die, and growth slows. When just as many cells are dying as are dividing, the batch enters the Stationary phase. This is the point at which the key nutrients are completely consumed, the fermentation is stopped and the broth is harvested. If the fermentation were allowed to continue, the cells would enter the Death phase.
The video resource "Advanced CPU Designs: Crash Course Computer Science #9" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.
After catastrophic flooding in New Orleans destroyed two hospitals, the Southeast Louisiana Veterans Health Care System is planning a replacement facility that will incorporate resilience against future extreme events.
Widespread damage from flooding at the Texas Medical Center in Houston revealed the complex's vulnerabilities. Implementing a long-term hazard mitigation plan is reducing future risks.
The video resource "Alan Turing: Crash Course Computer Science #15" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.
This site deals with issues related to individual rights and liberties. The site provides up-to-date cases that can be used for for the enhancement of criminal law and constitutional law students' research.
Panelists: Marick Lewis - Director, BioNetwork Pharmaceutical Center and Ryan Gilmore - Education Coordinator, BioNetwork Pharmaceutical Center Center BioNetwork's Analytical Training Lab: A Unique Resource for Industry, Instructors, and Students Located in the Piedmont Triad Research Park in downtown Winston-Salem, the Analytical Training Lab is the newest addition to the BioNetwork Pharmaceutical Center. The Lab contains analytical chemistry equipment used for industry training, as an instructional resource for college instructors, and for skill-building for students in physical or life science programs. Join us on a tour of this unique facility and learn how you can take advantage of this state-of-the-art resource.
The Purification process begins as the transfer tank of clarified lysate from the Recovery process is connected to the inlet pump on the Chromatography skid. The first Chromatography step in our GFP purification process is Anion-Exchange. At this point in the process, the pH of the clarified lysate is about eight-point-zero, which means that the protein is negatively charged. Because it is negatively charged, GFP will bind to the positively charged anion exchange resin. The pump draws the lysate from the vessel...past the first conductivity sensor and pressure sensor...and through the zero-point-four-five micron pre-filter. The pre-filter removes any residual cell debris or other particulates that may have contaminated the solution. If the pre-filter begins to clog, the pressure sensor at the inlet side of the filter will register a rising pressure...and the controller will signal the need for a filter change. After pre-filtering and before the column, the lysate passes through a flow meter... and an air sensor. Then, as the lysate passes over the resin beads, the negatively charged protein attaches to the positively charged beads. The solution leaving the column passes a UV optical density sensor, a conductivity sensor and a pH sensor. The optical density sensor's low readings confirm that the GFP is not in the solution, so the outlet valve sends the solution to waste.
Applying a simple stain to a bacterial culture is a technique that is used for examining the size, shape, and arrangement of a specimen. It uses a dye to stain the cells, making them easier to see under a microscope. In this experiment, we will demonstrate the steps for applying a simple stain to the bacterial organism, staphylococcus. Our hope is that after viewing this video, you will feel more confident and be better prepared to try applying a simple stain in a hands-on lab experience.
Brunswick Community College prepares students for jobs in the aquaculture and marine biotechnology industry. This brief video provides an overview of the aquaculture program.
Aquaculture is defined simply as the farming and husbandry of aquatic organisms. In practice aquaculture or mariculture (saltwater aquaculture) involves commercially growing seafood for consumers. It also involves the sciences of natural resource management and enhancement, collection methods, aquarium technology, facility construction and maintenance, and biotechnology production from marine organisms.
One of the primary safeguards that life science companies use to help prevent contamination in cleanroom operations is Gowning. Gowning is basically putting on clothing -- in this case layers of specially designed garments, covers, masks and gloves -- that isolates your body from the clean room environment. Gowning is more involved than just slipping on a coverall and going to work. Gowning is itself a process, with carefully sequenced steps to ensure that your body is fully covered, and that the protective garb remains free of contamination. In this video, we'll demonstrate the top-down approach for both general and aseptic gowning.
Presenter: Bill Terrill, Director of Industry Training, Wake Technical Community College This webinar provides information on why automation troubleshooting training and certification is needed, for whom it is designed as well as the concept, methodology, and use of mini-labs. In addition, the session includes a discussion of other training resources that are available for mechanics, electricians and technicians to meet certification standards.