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1 GFP overview
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In this program, we'll look at the production of Green Fluorescent Protein. GFP is used as a biological marker, and when attached to a drug it can provide researchers with a visual story of where the drug goes. It's a fluorescent dye that's very well tolerated by most cells and doesn't interfere with normal cellular function. Let's examine the 3 main phases of GFP production – Fermentation, Recovery, and Purification. Fermentation is basically cell farming. We program cells to produce a product, we nurture them as they grow and reproduce, and then we harvest them. In recovery we separate our product from the cells where they were housed. And then in purification we go a step further by removing everything else that's contaminating our product leaving us with a very pure, concentrated solution.

Subject:
Allied health personnel
Material Type:
Lesson
Date Added:
09/11/2018
21st Century Skills: Adaptability
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Change as an Opportunity
New Experiences
Considering Others' Viewpoints
Dealing with Stress

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Analysis/Solution Mindset
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Understanding Different Points of View
Understanding the Context of Problems
Connecting Information with Critical Thinking
Thinking About Multiple Solutions

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Collaboration
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Working as aTeam Player
Finding Common Ground
Shared Responsibilities and Creative Input
Dealing with Conflict in a Positive Way

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Communication
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Speaking Professionally
Communicating Digitally
Nonverbal Communication
Understanding How to Listen

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Digital Fluency
Unrestricted Use
Public Domain
Rating
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Inspiring Collaboration
Ethics and Innovation
Utilizing Technological Advances
Searching for Information

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Empathy
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Differentiating Empathy with Sympathy
Actively Listening
Respecting Diverse Backgrounds
Understanding Others' Needs at Work

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Entrepreneurial Mindset
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Taking Initiative
Connecting Ideas and Innovating
Taking Risks and Learning from Mistakes
Thinking Like an Entrepreneur

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Resilience
Unrestricted Use
Public Domain
Rating
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Being Prepared
Bouncing Back and Learning from Adversity
Dealing with Conflict and Compromise
Seeing Personal Growth as a Way of Life

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Self-Awareness
Unrestricted Use
Public Domain
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A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Evaluating Strengths and Areas of Growth
Understanding the Effects of Actions
Workplace Manners
Finding a Good Fit

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
21st Century Skills: Social Diversity/Awareness
Unrestricted Use
Public Domain
Rating
0.0 stars

A set of instructional videos paired with a simple assessment. Learners that get a passing score are awarded a digital badge.

Topics covered:

Respecting Other Backgrounds
Understanding the Importance of Social Diversity
Expanding Your Definition of "Normal"
Working Effectively in a Diverse Environment

Subject:
CTE
Material Type:
Assessment
Provider:
New World of Work
Date Added:
01/13/2021
2nd Centrifugation (GFP Recovery part 4 of 5)
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After the second homogenization, the lysed cell solution is pumped back through the centrifuge. But this time, our goal is different. Before lysing, our product was held within the E.coli cells. Now – with the cells broken apart, the cell contents - including cytoplasm and GFP - are mixed into the buffered solution. The centrifuge again spins out the solids - which are primarily cell debris - and it's the clarified liquid which contains our product – the GFP! This time we discard the solids and keep the liquid – which is now known as Lysate.

Subject:
Allied health personnel
Material Type:
Lesson
Date Added:
09/11/2018
3D Graphics: Crash Course Computer Science #27
Read the Fine Print
Educational Use
Rating
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The video resource "3D Graphics: Crash Course Computer Science #27" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

Subject:
Computer applications
Material Type:
Lesson
Date Added:
05/08/2018
The 4 Phases of Fermentation (GFP Fermentation part 1 of 6)
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Different cells have different needs. Some are aerobic – they need oxygen – while others are anaerobic and don't need oxygen. But the lifecycle of almost all cells follows a predictable 4 phase-pattern. Lag, Exponential, Stationary and Death. When a cell is first introduced to fresh growth media, it has to adapt to the new environment. This creates a lag in the growth timeline. After the organism adapts, the batch takes off. The cells begin dividing at a constant rate –an Exponential or Logarithmic increase. But eventually, the nutrients in the media are consumed, toxic metabolic waste products build up, cells begin to die, and growth slows. When just as many cells are dying as are dividing, the batch enters the Stationary phase. This is the point at which the key nutrients are completely consumed, the fermentation is stopped and the broth is harvested. If the fermentation were allowed to continue, the cells would enter the Death phase.

Subject:
Allied health personnel
Material Type:
Lesson
Date Added:
09/11/2018
Advanced CPU Designs: Crash Course Computer Science #9
Read the Fine Print
Educational Use
Rating
0.0 stars

The video resource "Advanced CPU Designs: Crash Course Computer Science #9" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

Subject:
Computer applications
Material Type:
Lesson
Date Added:
05/08/2018
After Katrina, Health Care Facility's Infrastructure Planned to Withstand Future Flooding
Read the Fine Print
Rating
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After catastrophic flooding in New Orleans destroyed two hospitals, the Southeast Louisiana Veterans Health Care System is planning a replacement facility that will incorporate resilience against future extreme events.

Subject:
Architecture
Design
Material Type:
Case Study
Provider:
National Oceanic and Atmospheric Administration
Provider Set:
U.S. Climate Resilience Toolkit
Date Added:
08/09/2016
After Record-Breaking Rains, a Major Medical Center's Hazard Mitigation Plan Improves Resilience
Read the Fine Print
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Widespread damage from flooding at the Texas Medical Center in Houston revealed the complex's vulnerabilities. Implementing a long-term hazard mitigation plan is reducing future risks.

Subject:
Architecture
Design
Material Type:
Case Study
Provider:
National Oceanic and Atmospheric Administration
Provider Set:
U.S. Climate Resilience Toolkit
Date Added:
08/29/2016
Alan Turing: Crash Course Computer Science #15
Read the Fine Print
Educational Use
Rating
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The video resource "Alan Turing: Crash Course Computer Science #15" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

Subject:
Computer applications
Material Type:
Lesson
Date Added:
05/08/2018
The American Civil Liberties Union (ACLU)
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This site deals with issues related to individual rights and liberties. The site provides up-to-date cases that can be used for for the enhancement of criminal law and constitutional law students' research.

Subject:
CTE
Material Type:
Lesson
Date Added:
11/12/2010