Panelist: Phillip (Skip) Kemp Aquaculture is the farming and husbandry of aquatic organisms. Carteret Community College aims to introduce and encourage aquaculture in the region and state as an innovative, practical and economically viable science of applied marine sciences. Topics of Discussion: 1. Farming includes propagation, spawning, culturing of juveniles and production of marketable products. 2. Husbandry includes handling and caring for the organisms during all or a portion of their life cycle. 3. Educational pathways to an aquaculture career. Career choices will be discussed for both aspects of the aquaculture program discussed and extensive footage of the hands-on techniques and training future workers receive will be shown.

This presentation will discuss different strategies being used today to quantify both plant and mammalian cells for cell culture in the bioprocessing industry or for research.

The premier site for any communicable disease research information, patient education or updates on public health and new findings.

The video resource "The Central Processing Unit (CPU): Crash Course Computer Science #7" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

This module includes, the basics of centrifugation, effects of gravity on particles in suspension, operation of a centrifuge, differential centrifugation, basic components, hazards and safety.

GFP Recovery begins with the arrival of the broth tank. A sterile hose is run from the broth tank to the Disk-Stack Centrifuge, and the tank is pressurized to drive the broth into the centrifuge. Once the centrifuge reaches a stable running speed...the inlet valve is opened and broth enters the bowl. The centrifugal force causes the solids to collect on the sides of the bowl - much like the spin cycle on your clothes washer. The liquid flows through and out of the centrifuge. As more broth enters the bowl, it displaces the clarified liquid to the top of the bowl where it exits, while the cells continue to build up on the bowl surface. The centrifuge has an integrated R-P-M monitor. If the unit is not rotating at a stable running speed, the controller will alarm and shut it down. The liquid leaving the bowl is known as the clarified stream, because almost all the solids have been removed. The solids are the E.coli cells, and they contain the product. A sensor monitors the clarified stream for percent solids. When the bowl has reached capacity for solids, the bowl opens and the solids are discharged into an appropriate container for collection. Once the solids are discharged, the centrifugation step can resume, while the clarified liquid is waste. At this point, the cells are in a paste form, and although most of the liquid has been removed, our cell paste is still about forty percent liquid by weight. The remaining liquid contains high levels of metabolites and salts that could complicate downstream processing, so we're going to lower those levels by washing the cells. The cell paste is suspended in a buffered solution and then run through the centrifuge again. As the clarified liquid leaves the centrifuge this time, it carries many of the contaminants from the fermentation step with it.

In this lesson, we'll learn about the 2 most important tools to identify chemical hazards: , Safety Data Sheets (SDS) and , Chemical Labels Many of the chemicals we use in the lab are potentially dangerous, especially under high heat, pressure, or when they're mixed with other chemicals.

This discussion was originally presented at the Plant Grafting Workshop hosted at Wilkes Community College in April 2009. In this video, Bill Hanlin of the Wilkes Cooperative Extension Service, explains a bit of the history behind plant grafting.

This module includes, basics of Chromatography, affinity, ion exchange, size exclusion, part and functions associated with chromatography.

This series of lectures was originally presented on January 9, 2009 at the "Particle Fundamentals Forum" hosted at BTEC on NCSU's Centennial Campus and is now available for you to see ON-DEMAND! You will learn about particles, what they are, how they are detected, forces on particles, and the types of instruments used to quantify these contaminants. Professionals with real-world experience will discuss on the mechanics of detecting particulate, the benefits and considerations of various monitoring techniques, and will also offer insight on the future trends associated with contamination and contamination control.

This plant grafting demonstration was originally presented at the Plant Grafting Workshop hosted at Wilkes Community College in April 2009. In this video, Ronald Dollyhite, Lead Instructor, Horticulture Technology at Wilkes Community College, demonstrates how to perform a cleft graft.

Panalists: Angela Kimel, Laurin Mancour, Jennifer Pullum, & Vernon Shoaf Are you thinking about starting a career in clinical research? Do you have a healthcare background? Are looking for new career opportunities? Do you want to learn more before beginning a degree program at a community college or university? November's BioForum places the spotlight on the growing clinical research industry in North Carolina. We'll discuss current and future developments in clinical research, educational opportunities across the state, and preview an exciting new online course that will debut in December. Foundations of Clinical Research is a 2-week online course designed to provide you with a fundamental understanding of the clinical research industry. This online course features highly interactive, scenario-based learning activities. You will work through each activity at your own pace and explore the key principles necessary to begin a career in clinical research.

Panelist: Holly Weir, Environment Sector Project Director, NCCCS Code Green Curriculum Improvement Project Workforce training in North Carolina is getting a "green" makeover. North Carolina's economy has changed to include a greater concentration of sustainability products, processes, practices, and technologies. North Carolina Community Colleges have emerged as a primary driver in the state's sustainability sector. The Code Green Super CIP aims to integrate sustainability skills across the curriculum beginning in the areas of energy, building, environment, transportation, and engineering technology. By incorporating sustainability skills and knowledge into curriculum programs, colleges are better able to prepare students for success in the 21st century workforce.

The video resource "The Cold War and Consumerism: Crash Course Computer Science #24" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

Learn how to collect and import spatial features from the field, use web-based map tools to engage citizens, and incorporate the best available spatial data from public domain sources.

In this class, get an overview of looking for a job online. We cover online classified sites, job search sites, company sites, and staying safe from scams.

The video resource "Compression: Crash Course Computer Science #21" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

The video resource "Computer Networks: Crash Course Computer Science #28" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

The video resource "Computer Vision: Crash Course Computer Science #35" is included in the "Computer Science" course from the resources series of "Crash Course". Crash Course is a educational video series from John and Hank Green.

We'll be using TFF for two different tasks within the purification process: Concentration and Diafiltration. As the solution moves through the TFF apparatus, it leaves the supply tank...is pulled through a pump...past a pressure sensor...and then across the filter membrane. Everything that passes through the membrane, including the buffer solution, is known as permeate – and for this process - is sent to waste. The GFP protein is larger than the pores of the membrane and is retained. The retained material – called Retentate – is recirculated to the supply tank. Recirculation of the feed continues until the desired concentration of GFP is achieved. Following concentration, and while the protein solution is recirculating, a new storage buffer solution is added to the feed. In effect, the protein is being washed by the flow of a new buffer solution in, and the old buffer solution out. As this diafiltration step proceeds, the buffer solution that's being added to the feed replaces the buffer solution that the GFP was originally in, effectively removing any remaining salt as well. When this process is complete, the GFP solution is routed through a point-two-two micron final filter...and then collected in appropriate containers – usually bottles or bags. The Purification process is complete. The Green Fluorescent Protein concentrate can now move downstream to final Fill-Finish to be freeze-dried and packaged.