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2nd Centrifugation (GFP Recovery part 4 of 5)
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After the second homogenization, the lysed cell solution is pumped back through the centrifuge. But this time, our goal is different. Before lysing, our product was held within the E.coli cells. Now – with the cells broken apart, the cell contents - including cytoplasm and GFP - are mixed into the buffered solution. The centrifuge again spins out the solids - which are primarily cell debris - and it's the clarified liquid which contains our product – the GFP! This time we discard the solids and keep the liquid – which is now known as Lysate.

Subject:
Allied health personnel
CTE
Material Type:
Lesson
Date Added:
09/11/2018
360 Essentials: A Beginner's Guide to Immersive Video Storytelling
Unrestricted Use
CC BY
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Our objective with this resource is to walk you through the essential steps in creating compelling and engaging 360 video experiences. While some prior experience with photography or videography can help, the technology available now gives anybody the ability to produce this type of amazing content.

Subject:
Arts and Humanities
Visual arts
Material Type:
Textbook
Provider:
Ryerson University
Author:
Adrian Ma
Gary Gould
Joshua Cameron
Date Added:
06/08/2021
3 Arguments Why Marijuana Should Stay Illegal Reviewed
Read the Fine Print
Educational Use
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We take a fair look at some of the best counter arguments for legalization and see how they hold up in review. The video "3 Arguments Why Marijuana Should Stay Illegal Reviewed" is a resource included in the Law topic made available from the Kurzgesagt open educational resource series.

Subject:
Law
Social and Behavioral Science
Date Added:
10/28/2020